Available: [7] B

(11)Fmoc-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (11) Preparation of Fmoc-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (10)Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin(20DMF)5203DMF3DMF2Fmoc-Pro-OH0.8gDIEA0.4mlTBTU0.56gHOBT0.2gDMF10ml1.53DMF3DMF2Fmoc-Pro-Gly-Lys(Boc)-Pro- Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin In step (10) of Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin, add decapping reagent (20% piperidine in DMF solution), reacted at room temperature for 5 minutes, drained, added decapping agent and reacted at room temperature for 20 minutes, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with re-distilled DMF, drained, added Fmoc-Pro- 0.8g of OH, 0.4ml of DIEA, 0.56g of TBTU, 0.2g of HOBT, and 10ml of DMF were reacted at room temperature for 1.5 hours, dried with a vacuum pump, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with distilled DMF, pumped Dry, obtain Fmoc-Pro-Gly-Lys (Boc)-Pro-Ala-Asp (otBu)-Asp (otBu)-Ala-Gly-Leu-Val-WangResin

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Adequate B12 levels have been known to help prevent anemia and major birth defects, may support bone health, may reduce the risk of macular degeneration, and even help with mood improvement and symptoms of depression